Figure 1.
![Figure 1. Deficiency of p85α in BMMs results in reduced proliferation. Nonadherent bone marrow cells (1 × 106) from WT and p85α–/– mice were cultured in a 24-well tissue-culture plate in the presence of M-CSF (100 ng/mL) for 3 days. Cells were starved overnight using IMDM and stimulated with either M-CSF (100 ng/mL) or GM-CSF (50 ng/mL) for 24 hours. Proliferation was measured by thymidine incorporation assay. Bars represent the mean thymidine incorporation in BMMs in response to either M-CSF or GM-CSF stimulation (counts per minute [cpm] ± SD) of 2 experiments performed in duplicate. Similar results were observed in 3 independent experiments. *P<.05 for WT versus p85α–/–.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/1/10.1182_blood-2004-10-4041/4/zh80130580760001.jpeg?Expires=1735591951&Signature=BQFiVWfX954gDJBIT0dO6PiHjoHLqhAJlKLeU8o4D-otRiX4oMWd5nN7FnNLqGc2JzOrDT6MPZjZcujCAUROB-yx-L49m4CHb5WrCuWBEr4vfZJ2vvaLGTSlAQD4Z59TXbC2-I6REX5ZUWPUAxRN~QWQ0XO-xcNay-GZEilqjlpuBdtP7Bq811dbz8g4ScUXouO7XfqBbg0fLVZ-kn6TWriyCym~vDtufVtUaknDpDXUmSz3rrJUnkxpCkcmmgxDk8aX8FrXIL1~KdW7P3TgMiua8eB2cJ3q6MqWhDgEPmc39dXZNR-qj0X-a71SK4krMUP4iN6c948Emgkc50VKiA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Deficiency of p85α in BMMs results in reduced proliferation. Nonadherent bone marrow cells (1 × 106) from WT and p85α–/– mice were cultured in a 24-well tissue-culture plate in the presence of M-CSF (100 ng/mL) for 3 days. Cells were starved overnight using IMDM and stimulated with either M-CSF (100 ng/mL) or GM-CSF (50 ng/mL) for 24 hours. Proliferation was measured by thymidine incorporation assay. Bars represent the mean thymidine incorporation in BMMs in response to either M-CSF or GM-CSF stimulation (counts per minute [cpm] ± SD) of 2 experiments performed in duplicate. Similar results were observed in 3 independent experiments. *P<.05 for WT versus p85α–/–.
