American Society of Hematology

Figure 2.

$Figure 2. RT-PCR analysis of GALVR, RDR, and FLVCR mRNA levels in human bone marrow and hematopoietic progenitor and stem cells. RNA from FACS-sorted bone marrow fractions was analyzed by RT-PCR. The data for unfractionated (Unfr.) bone marrow was normalized to the data from Northern blot analysis of the same RNA samples as in Figure 1. The level of GALVR mRNA in unfractionated bone marrow was set as 1 and compared with the relative amount of RDR and FLVCR mRNA in unfractionated bone marrow, Lin– CD34+ CD38+, and Lin– CD34+ CD38– cells. *Significantly higher than the level of GALVR mRNA (P < .001). **Significantly higher than the level of GALVR mRNA (P < .001); significantly lower than the level of FLVCR mRNA (P < .001). Error bars indicate standard deviation.$

RT-PCR analysis of GALVR, RDR, and FLVCR mRNA levels in human bone marrow and hematopoietic progenitor and stem cells. RNA from FACS-sorted bone marrow fractions was analyzed by RT-PCR. The data for unfractionated (Unfr.) bone marrow was normalized to the data from Northern blot analysis of the same RNA samples as in Figure 1. The level of GALVR mRNA in unfractionated bone marrow was set as 1 and compared with the relative amount of RDR and FLVCR mRNA in unfractionated bone marrow, Lin^– CD34⁺ CD38⁺, and Lin^– CD34⁺ CD38^– cells. *Significantly higher than the level of GALVR mRNA (P < .001). **Significantly higher than the level of GALVR mRNA (P < .001); significantly lower than the level of FLVCR mRNA (P < .001). Error bars indicate standard deviation.

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