Figure 4.
Expression of chimeric receptors by NK cells expanded from peripheral blood mononuclear cells. (A) Surface receptor expression was visualized by flow cytometry after staining with a goat anti-mouse (Fab)2 polyclonal antibody conjugated with biotin followed by streptavidin PerCP (y-axes); expression of GFP is also shown (x-axes). (B) Western blot analysis of chimeric receptor expression in NK cells, under reducing or nonreducing conditions. Filter membranes were labeled with an antihuman CD3ζ antibody and a goat anti-mouse IgG horseradish peroxidase-conjugated second antibody.
