![Figure 1. Immunohistochemistry and immunofluorescence in PCNSL and peripheral diffuse large B-cell lymphoma stained with anti-CXCR4 antibody. Immunohistochemistry and immunofluorescence demonstrate the cytoplasmic expression of CXCR4 in PCNSL (A-C) and combined cytoplasmic-membranous staining of CXCR4 in a peripheral diffuse large B-cell lymphoma (D-F). (A,D) CXCR4 staining using conventional immunohistochemistry; the arrow indicates membranous staining (APAAP; original magnification, × 600 using an Olympus AX70 microscope [Olympus, Melville, NY]). (B,E) CXCR4 staining by immunofluorescence (Cy2 labeled); (C,F) combined CXCR4/DAPI immunofluorescence staining (original magnification, ×100 under a 1.30 UPlan FL oil objective lens used with an Olympus BX60 microscope). A ColorView II camera and AnalySIS software (Soft Imaging Systems, Muenster, Germany) were used for image acquisition and processing.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/1/10.1182_blood-2005-01-0324/4/zh80130580750001.jpeg?Expires=1735573938&Signature=xyVJ65~Ek4aVD~zr0waeYeKKrVS-25H8XKFYFT1tjNHJc4bIAzQsl7UtYsWR2AglJrOFuowqsHZbBCrq~lX6Ur07MQWuhrUCfpcUokCsOrd~2hqVQDeHIC862oF2Vkwd6BjPIlcdftncLOLz8oLJz5uMnF3HVVdWhVD4CaOwEg63WRfZrZdK4M5n-oRyWflSruSqx6FfQi8RMAGaoukfDqb0wF1uEcYcmQuJ8hjqll8wWQKipRBPugaq5XQ-GTx5h1vimTyQYzExWvFymTSGEtdfkxbzj4yzw9zBnhqMh6~yO2fFD3zU6~UsreJhWqkAutnSM5mI8z4EkVDydklzRA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunohistochemistry and immunofluorescence in PCNSL and peripheral diffuse large B-cell lymphoma stained with anti-CXCR4 antibody. Immunohistochemistry and immunofluorescence demonstrate the cytoplasmic expression of CXCR4 in PCNSL (A-C) and combined cytoplasmic-membranous staining of CXCR4 in a peripheral diffuse large B-cell lymphoma (D-F). (A,D) CXCR4 staining using conventional immunohistochemistry; the arrow indicates membranous staining (APAAP; original magnification, × 600 using an Olympus AX70 microscope [Olympus, Melville, NY]). (B,E) CXCR4 staining by immunofluorescence (Cy2 labeled); (C,F) combined CXCR4/DAPI immunofluorescence staining (original magnification, ×100 under a 1.30 UPlan FL oil objective lens used with an Olympus BX60 microscope). A ColorView II camera and AnalySIS software (Soft Imaging Systems, Muenster, Germany) were used for image acquisition and processing.
