Figure 1.
Figure 1. Contribution of the nonleukocyte component of class-Ib PI3K activity to neutrophil accumulation in response to LPS. GFP-expressing cell counts per unit area obtained 6 hours after intratracheal administration of LPS (10 μg/g) in mice (A) deficient in or chimeric for PI3Kγ activity, (B) deficient in or chimeric for PI3Kδ activity, (C) pretreated with the function-blocking or nonfunction-blocking F(ab')2 9A9 and CL37, respectively. (A) ▪ indicates WT littermate (GFP–/+ p110γ+/+); ▪, GFP–/+ p110γ–/–; and ▨, p110γ–/– reconstituted with GFP–/+ p110γ+/+ fetal liver cells. (B) ▪ indicates WT littermate (GFP–/+ p110δ+/+); ▪, GFP–/+ p110δ–/–; and ▨, p110δ–/– reconstituted with GFP–/+ p110δ+/+ fetal liver cells. (C) ▪ indicates WT littermate (GFP–/+ p110δ+/+); ▪, WT littermate plus mAB CL37; and ▨, WT littermate plus mAb 9A9. Mean values (± SD) are shown for 8 mice in each experimental or control group. *P < .05.

Contribution of the nonleukocyte component of class-Ib PI3K activity to neutrophil accumulation in response to LPS. GFP-expressing cell counts per unit area obtained 6 hours after intratracheal administration of LPS (10 μg/g) in mice (A) deficient in or chimeric for PI3Kγ activity, (B) deficient in or chimeric for PI3Kδ activity, (C) pretreated with the function-blocking or nonfunction-blocking F(ab')2 9A9 and CL37, respectively. (A) ▪ indicates WT littermate (GFP–/+p110γ+/+); ▪, GFP–/+p110γ–/–; and ▨, p110γ–/– reconstituted with GFP–/+p110γ+/+ fetal liver cells. (B) ▪ indicates WT littermate (GFP–/+ p110δ+/+); ▪, GFP–/+ p110δ–/–; and ▨, p110δ–/– reconstituted with GFP–/+p110δ+/+ fetal liver cells. (C) ▪ indicates WT littermate (GFP–/+p110δ+/+); ▪, WT littermate plus mAB CL37; and ▨, WT littermate plus mAb 9A9. Mean values (± SD) are shown for 8 mice in each experimental or control group. *P < .05.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal