Pre-T (mice nos. 1149 and 1901) or megakaryocytic (mouse no. 1017) leukemia in NHD13 transgenic mice. (A) Bone marrow from mouse no. 1901 infiltrated with lymphoblasts characterized by high nuclear-cytoplasmic ratio, variably condensed nuclear chromatin, and prominent nucleoli, stained with MGG. (B-C) Perivascular infiltration of kidney from mouse no. 1901 with lymphoblasts, stained with H&E (B) or anti-CD3 (C). (D) Genomic DNA from control (lane 1) or pre-T LBL (mice nos. 1149 and 1901, lanes 2-3) mice digested with SstI and hybridized to a T-cell receptor β (TCRβ) probe. Clonal rearrangements are indicated with arrows. (E) Cells from mouse no. 1901 with pre-T LBL (thymus, spleen, and bone marrow) were stained with CD4 and CD8 and analyzed by fluorescence activated cell sorting (FACS). (F) Peripheral blood (from mouse no. 1017) smear showing dramatically increased platelet count, giant platelets, and megakaryoblasts, stained with MGG. (G) Osteosclerotic bone marrow (from mouse no. 1017) stained with H&E. (H) Liver infiltrated with megakaryocytes and megakaryoblasts, stained with H&E (inset, × 400) or anti-CD41.