NHD13 inhibits megakaryocytic differentiation. (A-B) Control transfectant pzsv5, untreated (A) or treated (B) with TPA. Multinucleated cells are indicated with arrows. (C-D) Stable transfectant that expresses NHD13 (pzsvNHs1), either untreated (C) or treated (D) with TPA. (E) Percentage of differentiating cells (mean and SD of 3 independent experiments). Pzsv5 and pzsv6 are control transfectants; pzsvNHs1 and pzsvNHs2 are stable NHD13 transfectants. (F) RT-PCR for PKC-ϵ and β-actin. (Top) PKC-ϵ, (bottom) β-actin. The numbers below the picture indicate the fold increase of PKC-ϵ expression normalized to β-actin expression.