Figure 4.
Figure 4. Expression of HAS1 and novel variant genes and proteins in MM cell lines. Western blot analysis. Protein lysates of MM cell lines were separated on SDS-PAGE, blotted onto nitrocellulose, and probed with (A) anti-HAS1 antibodies, (B) preincubated anti-HAS1 with blocking peptide, and (C) before bleed (preimmume serum). Arrows identify HAS1Va, about 35.9 kDa; HAS1Vb, about 39.5 kDa; and HAS1, about 65 kDa bands. The size of HAS1Va (about 35.9 kDa) and HAS1Vb (about 39.5 kDa) proteins were predicted using the ExPASy Molecular Biology Server. The anti-HAS1 specificity was evaluated by preincubating anti-HAS1 serum with the blocking peptide overnight before probing the membrane. The extra bands presented on the blot most likely are bands corresponding to HAS1 proteins encoded by other yet to be identified variants, and/or these bands represent the HAS proteins subjected to posttranslation modifications and glycosylation.

Expression of HAS1 and novel variant genes and proteins in MM cell lines. Western blot analysis. Protein lysates of MM cell lines were separated on SDS-PAGE, blotted onto nitrocellulose, and probed with (A) anti-HAS1 antibodies, (B) preincubated anti-HAS1 with blocking peptide, and (C) before bleed (preimmume serum). Arrows identify HAS1Va, about 35.9 kDa; HAS1Vb, about 39.5 kDa; and HAS1, about 65 kDa bands. The size of HAS1Va (about 35.9 kDa) and HAS1Vb (about 39.5 kDa) proteins were predicted using the ExPASy Molecular Biology Server. The anti-HAS1 specificity was evaluated by preincubating anti-HAS1 serum with the blocking peptide overnight before probing the membrane. The extra bands presented on the blot most likely are bands corresponding to HAS1 proteins encoded by other yet to be identified variants, and/or these bands represent the HAS proteins subjected to posttranslation modifications and glycosylation.

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