Figure 1.
![Figure 1. Generation of L-selectin-deficient lymphoma cells. (A) Genotype of T-lymphoma cell lines generated from normal (164T2 and 267), heterozygous (1681), and L-selectin-deficient mice (KOL lines) showing the presence of the wild-type allele (728 base pair [bp]) and the puromycin-containing mutant alleles (320 bp). (B) Surface expression of L-selectin on lymphoma cell lines. Cells were stained for L-selectin using the MEL-14 mAb by flow cytometry. Controls included cells incubated with SA-PE alone. The T-cell origin of all lymphoma cell lines was established by their ability to express CD3ϵ (data not shown). The x axis measures fluorescence intensity; the y axis, cell counts.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/12/10.1182_blood-2004-04-1406/5/zh80120580040001.jpeg?Expires=1735843077&Signature=XK26Pwab8WKPng6iPZYoqZNsOdbRlXW5qRJe6M1eAvZGGqTHVQLmcufKesA2gbCfh-vMDvWEQfH-q2jY3cni9JigpG7-cvDPmo-Xv85OFGXBvpkAZ0RNSKSc9Lp-VvJlGIgKpNH91YkO3TtoJ3AOqAW0jq7IOXIVEc44U-Byuzvanq-zfP~uvNS4qzC6MVTHixK~bXaD~TQlCh0FVqQ6g6atn9nlr5-0Zyj6UmmzvrzZbZBH7oSv1XEq4diDvaegB8wDzqvqsFQpLBhL86E2ztQWbn5rN9GXs4vTfWSB9C2kZs7U27RtfAoBA2SEBY~5auv75yaPtO1wJ8i0KTeIfw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Generation of L-selectin-deficient lymphoma cells. (A) Genotype of T-lymphoma cell lines generated from normal (164T2 and 267), heterozygous (1681), and L-selectin-deficient mice (KOL lines) showing the presence of the wild-type allele (728 base pair [bp]) and the puromycin-containing mutant alleles (320 bp). (B) Surface expression of L-selectin on lymphoma cell lines. Cells were stained for L-selectin using the MEL-14 mAb by flow cytometry. Controls included cells incubated with SA-PE alone. The T-cell origin of all lymphoma cell lines was established by their ability to express CD3ϵ (data not shown). The x axis measures fluorescence intensity; the y axis, cell counts.
