FGFR3 immunoreactivity and OS. (A) Detection of FGFR3 and t(4;14) in MM. Immunohistochemistry study with anti-FGFR3 antibody showed a positive reaction with the tumor tissue from the t(4;14) KMS11 cell line–injected mouse (i), a negative reaction with a bone marrow biopsy from a t(4;14)-negative myeloma patient (ii), and a positive reaction with a bone marrow biopsy from a t(4;14)-positive myeloma patient (iii). cIg-FISH showed a fusion signal indicating a t(4;14) translocation in a myeloma cell (iv). (B) OS of MM patients according to FGFR3 expression by IHC. The image in panel iv was obtained with an Axioscop microscope equipped with UPlanFl 20 ×/0.40 and 40 ×/0.75 objective lenses (Zeiss, Jena, Germany).