Characterization of the cells in the pouch fluid. The cells in the pouch fluid were collected from both MGL1-deficient (closed square in G) and wild-type (open square in G) mice on days 1 to 5, and the number of cells was counted (G). Using the cells on day 4 from both wild-type (A-C) and MGL1-deficient mice (D-F), the expression of MGL1/2, CD11b, CD45, F4/80, or moma-2 was analyzed by flow cytometry. Flow cytometric scatter profiles of the cells are shown in panels A and D (SS, side scatter; FS, forward scatter). The expressions of MGL1/2, CD11b, or CD45 on the cells in panel A or D are shown in panels B and E. Open histograms in panels B and E represent staining with control mAb. The expressions of MGL1/2, CD11b, CD45, F4/80, or moma-2 on cells in the subpopulation circled in panel A (wild-type) and panel D (MGL1-deficient) are shown in panel C and panel F, respectively. The number of cells recovered from the pouch fluid of MGL1-deficient mice was greater than that recovered from wild-type mice. Furthermore, there was a predominant subpopulation having a unique cell surface phenotype of CD11b-negative/CD45-negative/MGL1/2-negative/F4/80-negative/moma-2–negative in MGL1-deficient mice.