Characterization of 2 populations of thrombocytes in zebrafish. (A) The subpanels correspond to (i) confocal images of zebrafish thrombocytes using bright field, (ii) B-2 filter (showing DiI fluorescence), (iii) G-2A filter (showing mepacrine fluorescence), and (iv) the composite of images (orange shows dual labeling by DiI and mepacrine) acquired by B-2 and G-2A filters. (B) The panels show forward and side scattering of FACS-sorted, mepacrine-labeled (green) and DiI-labeled (red) thrombocytes, respectively. (C) Top and bottom panels represent electron micrographs of FACS-sorted, DiI-labeled and mepacrine-labeled thrombocytes, respectively (original magnifications: left panels, ×18 000; right panels, ×36 000).