Representative dot plots demonstrating presence of GPIIb-positive cells in whole blood detected by the use of a polyclonal antibody against human GPIIB and a TRITC-conjugated second antibody. All cell populations were included in the gate. Graphs depict (A) unstained control, (B) IgG control, (C) second antibody alone control, and (D) GPIIb antibody staining. The TRITC fluorescence of the second antibody was measured in FL2 channel shown on the y-axis, and the percentages of GPIIb-positive cells are shown above the horizontal line.