Effect of rabbit Abs raised against HIV-1 10-mer wild-type peptides or 7-mer phage peptides on platelet fragmentation and oxidation. (Top) Gel-filtered platelets were incubated with control rabbit IgG (upper left quadrant) or rabbit Ab against peptide batches 1096 (upper right quadrant), 1098 (lower left), and 1099 (lower right) at 37°C for 4 hours. The percentages refer to fragmented platelets per 10 000 events counted. (Bottom) Similar incubation with assay of Ab-induced platelet oxidation. Ct indicates control buffer; Ci, control IgG; and Pt, patient IgG. Ca and DP indicate preincubation with scavenger of reactive oxygen species and inhibitor of NADPH oxidase, catalase (60 μg/mL) and diphenylenediodonium (100 nM), respectively; 96 and 98 refer to rabbit Ab against peptide batches 1096 and 1098.