American Society of Hematology

Figure 4.

$Figure 4. CD16 stimulation induces membrane-associated PI5KIα activation. (A) PI5KIα was immunoprecipitated from membrane and soluble fraction of unstimulated or CD16-stimulated cultured NK cells. Immunoprecipitates were assayed for PI5KIα activity, and the reaction products were subjected to TLC followed by autoradiography. Anti–MHCI-stimulated samples (not shown) showed a kinase activity that was superimposable to the unstimulated sample. (B) An aliquot of the immunoprecipitates from the same experiment was analyzed by immunoblot with anti-PI5KIα Ab. (C) The ratio between kinase activity and the amount of the kinase precipitated from the samples of the same experiment obtained by densitometric analysis, is shown. One representative experiment of 3 performed is shown.$

CD16 stimulation induces membrane-associated PI5KIα activation. (A) PI5KIα was immunoprecipitated from membrane and soluble fraction of unstimulated or CD16-stimulated cultured NK cells. Immunoprecipitates were assayed for PI5KIα activity, and the reaction products were subjected to TLC followed by autoradiography. Anti–MHCI-stimulated samples (not shown) showed a kinase activity that was superimposable to the unstimulated sample. (B) An aliquot of the immunoprecipitates from the same experiment was analyzed by immunoblot with anti-PI5KIα Ab. (C) The ratio between kinase activity and the amount of the kinase precipitated from the samples of the same experiment obtained by densitometric analysis, is shown. One representative experiment of 3 performed is shown.

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