Figure 1.
5-lipoxygenase metabolites dose dependently increase microbicidal activity in AMs infected with opsonized K pneumoniae. Rat AMs were infected with 50:1 opsonized K pneumoniae. Thirty minutes after infection, the cells were or were not (horizontal dotted line) incubated with LTB4, LTC4, LTD4, or 5-(S) HETE at the indicated concentrations. Microbicidal activity was assessed colorimetrically as described in “Materials and methods.” Data are expressed as the mean ± SE percentage survival of ingested bacteria from 3 independent experiments, each performed in triplicate. *P < .05 versus control by ANOVA.
