Analysis of unsorted and CD34⁺/CD38^--selected cells from AML patient samples. Eight AML samples, all harboring FLT3/ITD mutations, were stained with anti-CD34 and anti-CD38 fluorochrome-conjugated antibodies and subjected to immunomagnetic cell separation, as described in “Materials and methods.” The dot plots from the analysis of each sample are displayed above, with each patient represented by a row. The y-axis of all plots represents CD34 staining. The first column represents the unsorted cells and the middle column is the analysis of the CD34⁺/CD38^--selected cells. The column on the right represents the CD135 staining of the selected cells. All analyses shown were performed without gates. The quadrant markers in the middle row were used to obtain the percentages listed in Table 2.