Defective Vav expression in T-CVID T cells. Anti-Rac (A) or anti-Vav (B) immunoblots of postnuclear supernatants of PBL from patients with T-CVID (T-CVID1-4), 1 or 2 disease control patients (dis.ctr1 and 2), and a representative healthy control (ctr; n ≥ 4). Filters were stripped and reprobed with antiactin mAb. Migration of molecular mass markers is indicated. (C-D) Semiquantitative RT-PCR analysis of VAV1 (C) or of VAV2 and VAV3 (D) mRNA levels in T-CVID PBLs. VAV-specific RT-PCR products from patients with T-CVID (T-CVID1-4), 1 disease control patient (dis.ctr1), and a pool of 3 healthy controls (ctr) were resolved by agarose gel electrophoresis, quantitated by laser densitometry, and normalized to the levels of GAPDH mRNA used as internal control. Data are expressed as percentages of the levels of Vav mRNA in PBLs pooled from 3 healthy controls. Raw data of a representative experiment (n ≥ 2) are shown in the inset. Error bars represent SD.