Expression of CD180 and TLR4 in WT and Vav1/2^-/- mice. (A) Five percent probability contour plots showing CD21 and CD23 surface staining of B220⁺ gated splenocytes from WT and Vav1/2^-/- mice. MZ B cells fall within the CD21^high CD23^low gate, and the percentage of MZ and FO B cells are indicated. SSC indicates side scatter. (B) Expression of CD180 on FO and MZ B cells from WT and Vav1/2^-/- mice using the gating from panel A. (C) Marginal zone and follicular B cells from WT or Vav1/2^-/- mice were FACS sorted according to CD21 and CD23 levels as described in panel A. Expression of TLR4 was measured by real-time PCR on cDNA from the indicated populations; WT mice (□) and Vav1/2^-/- mice (▪). No significant differences were observed between the groups. Error bars indicate standard deviation.