Figure 4.
![Figure 4. CKLiK activity induced by inflammatory mediators. Peripheral blood eosinophils (A) and neutrophils (B-C) were isolated and stimulated with PAF (10-6 M), fMLP (10-6 M), or PMA (100 ng/mL), respectively. Cells were lysed (3 × 107 cells/point) and immunoprecipitated with CKLiK antibody, and kinase assays were performed without addition of Ca2+ and calmodulin, except for positive controls. (D) Ca2+ response of neutrophils to fMLP. Cells were loaded with Fura-2AM for 15 minutes and washed. Changes in [Ca2+]i were measured at 340 nm (F1) and 380 nm (F2) at 510 emission using a Hitachi F4500 fluorescence spectrophotometer. Similar Ca2+ responses were obtained after PAF stimulation of eosinophils (data not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/3/10.1182_blood-2004-09-3755/6/zh80150581690004.jpeg?Expires=1743289416&Signature=gtiknPFH0D~ddPgPawgZda46Xuygn2MjpLh3fWOFwOmWNvsbQ~JsMcXaFwKVQZHFz7HvPkDrFpYdlwbR1Zj-N-JVI6Nfa0jHJzTIUwLvihB-2hsZu1bFWM0LlX80j69AsgYT7IXUgasRdsAELz-~AAs7D0CT99~zDo34OfQ99aY3FsZybFoY2iEkDOy6bl6y44tdYCz5~ZI1jx-6myxhFb9Qgcikzv1eaxntfomkzesrhzwYJWAy33~Hv7tJg-7ZQGOtU9ToMUD2bc0oZ-i93UV-khK4SU2P43Y6lFM1bxMF7ig0nX~KvBkWjIGsbiZ476C0hy0XQZbtfhBdlLogug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CKLiK activity induced by inflammatory mediators. Peripheral blood eosinophils (A) and neutrophils (B-C) were isolated and stimulated with PAF (10-6 M), fMLP (10-6 M), or PMA (100 ng/mL), respectively. Cells were lysed (3 × 107 cells/point) and immunoprecipitated with CKLiK antibody, and kinase assays were performed without addition of Ca2+ and calmodulin, except for positive controls. (D) Ca2+ response of neutrophils to fMLP. Cells were loaded with Fura-2AM for 15 minutes and washed. Changes in [Ca2+]i were measured at 340 nm (F1) and 380 nm (F2) at 510 emission using a Hitachi F4500 fluorescence spectrophotometer. Similar Ca2+ responses were obtained after PAF stimulation of eosinophils (data not shown).
