American Society of Hematology

Figure 6.

$Figure 6. Elevated ICAM-1 expression does not trigger T-lymphocyte transcellular TEM. The site of T-lymphocyte arrest and subsequent TEM, or PMN location of TEM across 4-hour TNF-α-activated iHUVECs or ICAM-1 GFP iHUVECs was determined by live-cell imaging as in the legend of Figure 4. (A) The percentage of T lymphocytes that transmigrated by paracellular or transcellular routes was determined. (B) The fraction of transmigrated T lymphocytes with different sites of arrest and TEM of ICAM-1GFP iHUVECs, grouped as in Figure 4A, were determined. (C) Four-hour TNF-α-activated ICAM-1GFP iHUVECs were incubated for 15 minutes with 100 ng/mL recombinant human IL-8, 10 μg/mL SDF-1α, or buffer alone and then assayed for PMN TEM as detailed in the legend of Figure 4.$

Elevated ICAM-1 expression does not trigger T-lymphocyte transcellular TEM. The site of T-lymphocyte arrest and subsequent TEM, or PMN location of TEM across 4-hour TNF-α-activated iHUVECs or ICAM-1 GFP iHUVECs was determined by live-cell imaging as in the legend of Figure 4. (A) The percentage of T lymphocytes that transmigrated by paracellular or transcellular routes was determined. (B) The fraction of transmigrated T lymphocytes with different sites of arrest and TEM of ICAM-1GFP iHUVECs, grouped as in Figure 4A, were determined. (C) Four-hour TNF-α-activated ICAM-1GFP iHUVECs were incubated for 15 minutes with 100 ng/mL recombinant human IL-8, 10 μg/mL SDF-1α, or buffer alone and then assayed for PMN TEM as detailed in the legend of Figure 4.

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