Figure 6.
In the presence of allogeneic T cells, OPN augments the production of IL-12p70 by DCs and IFN-γ by T cells. MLR was performed with immature DCs (10 × 103/200 μL), cultured for 5 days prior to MLR and allogeneic T cells (10 × 104/200 μL) in the presence or absence of OPN, added throughout the MLR (A,C). (B,D) DCs were prestimulated with OPN from day 5 to day 7 of DC culture or left untreated as controls prior to addition to MLR. Supernatants were obtained after 48 hours of coculture and analyzed for their content of IL-12p70 and IL-10 (A-B), or IFN-γ and IL-13 (C-D) by ELISA. Data are given as mean pg/mL ± SD of duplicate measurements. Shown is 1 representative of 7 independent experiments.
