Characterization of tissue pancreatic MSCs. (A) Surface marker profile of pancreatic MSCs after 3 weeks of culture (top row) and BM-MSCs (bottom row). Open curves refer to control antibody signal; shaded curves refer to specific antibody signal. Results are from 1 experiment of 3 performed. (B) Morphology of BM-MSCs and tissue pancreatic MSCs. Both MSCs appeared adherent in monolayer with a fibroblast-like morphology. (C) Osteogenic differentiation of tissue pancreatic MSCs (“Materials and methods”). Tissue pancreatic MSCs in culture without differentiation medium (left) and with differentiation medium (middle and right). After differentiation, cells stained positive for alizarin red (right). (D) Surface expression of CCR1, CCR7, CXCR4, CXCR6, and CX3CR1 on tissue pancreatic MSCs detected by flow cytometry. (E) The percentage of positive cells for the chemokine receptors is reported (mean ± SD; n = 2) and compared with the values obtained for BM-MSCs.