JAM-A mRNA expression. Representative RT-PCR gels (left) and the results of densitometric analysis presented as ratio of JAM-A to β-actin (right) show mRNA expression of JAM-A in hepatic tissue of sham-operated mice and mice after 90 minutes of hepatic ischemia and 140 minutes of reperfusion. The number of cycles was 35 for JAM-A and 23 for β-actin. n = 6 animals per group; mean ± SEM.