Figure 3.
Tpo-induced expansion of hematopoietic clusters and myeloid progenitors derived from B-raf–/– ES cells cocultured with OP9 cells. The number of (A) day-8 hematopoietic clusters and (B) day-9 myeloid progenitors generated from 1 × 105 day-5 mesodermal colony cells in the presence or absence of TPO (50 ng/mL from day-5 replating onward) are presented. (C) The fold expansion, calculated by dividing the number of clusters and progenitors generated in the presence of TPO by those generated in the absence of TPO, is plotted. (A-C) The data represent the average ± SD for 3 independent experiments.
