Figure 3.
Figure 3. Role of Gαq in tyrosine phosphorylation of PKCδ in platelets. Platelets from mice deficient in the Gαq protein (A) or from wild-type mice (B) were stimulated with AYPGKF (500 μM) at 37°C, and the reaction was stopped by adding the cell lysis buffer. PKCδ was immunoprecipitated as described, and the samples were analyzed for tyrosine and threonine 505 phosphorylation by Western blotting using the monoclonal phosphotyrosine (4G10) and phospho-PKCδ (T505) antibodies, respectively. Equal lane loading was assured by probing the samples with PKCδ antibody. The Western blot shown is representative of experiments done using platelets from 3 different donors.

Role of Gαq in tyrosine phosphorylation of PKCδ in platelets. Platelets from mice deficient in the Gαq protein (A) or from wild-type mice (B) were stimulated with AYPGKF (500 μM) at 37°C, and the reaction was stopped by adding the cell lysis buffer. PKCδ was immunoprecipitated as described, and the samples were analyzed for tyrosine and threonine 505 phosphorylation by Western blotting using the monoclonal phosphotyrosine (4G10) and phospho-PKCδ (T505) antibodies, respectively. Equal lane loading was assured by probing the samples with PKCδ antibody. The Western blot shown is representative of experiments done using platelets from 3 different donors.

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