Figure 4.
Ly49 and CD94/NKG2 receptor expression on NK cells after transfer of wild-type lymphoid progenitor cells into LTβR–/–mice. Sorted lymphoid-committed progenitor cells from congenic B6-CD45.1 mice were injected intravenously in irradiated B6 (n = 3) and LTβR–/– (n = 4) mice, which both express the CD45.2 allele. Four to 5 weeks after injection flow cytometric analysis was performed. DX5-enriched splenocytes from both strains were stained with mAbs for NK1.1, CD3, CD45.1, and the indicated Ly49 receptors or CD94/NKG2. Expression percentages of the indicated Ly49 receptors and CD94/NKG2 were obtained by gating on CD45.1+NK1.1+CD3– NK cells. *P < .05 by Student t test.