WT1 peptide–specific cytokine production by TCR gene–transduced Th1 and Tc1 cells. TCR gene–transduced Th1 and Tc1 cells (1 × 10⁵), obtained from nonspecific Th1 and Tc1 cells of the same healthy donor of T cells used in the experiments of Figure 3, were cocultured with WT1 peptide–pulsed, CMV peptide–pulsed, or unloaded HLA-A24⁺ LCLs (TAK-LCL) (5 × 10⁴). After 20 hours, supernatants were harvested from culture, and their IFN-γ (A) and IL-2 levels (B) were determined by ELISA. UD represents undetected. Similar results were obtained using TCR gene–transduced Th1 and Tc1 cells derived from 2 other HLA-A24⁺ and 2 HLA-A24^– healthy volunteers. Error bars indicate standard error (SE) in triplicate samples.