Clinical development or disappearance of trisomy 8 associated with presence or absence of Vβ expansion. (A) Flow cytometric examination of Vβ subfamilies from patients 5, 7, and 8 before and after IST. Values for each patient (light bars) are superimposed on mean values of normal cohort (dark bars). (B) FISH data showing percentage of trisomy 8 before and after IST. (C) Effect of lymphocytes on trisomy 8 cell growth before and after IST. Paired lymphocytes and BMMCs obtained before and after IST were incubated in close contact with effector cells (with effector-target ratios of 2:1) for 2 hours before placement in short-term culture for 14 days. When FISH was performed, the number of trisomy 8 cells was decreased by cocultivation with the lymphocytes obtained before IST only.