xNAP1L depletion causes reduction of blood but not endothelial marker expression in stage-17 embryos. In all panels, control MO–injected embryos are on the left and xNAP1L MO–injected embryos, on the right. Embryos were injected with 12 ng of the appropriate MO at the single-cell stage and allowed to develop to stage 17 when they were fixed and marker-gene expression was analyzed by in situ hybridization of SCL, Xaml, xHex, or Fli1. Ventral views of embryos show expression in the precursors of the VBI, and Xaml expression in neurons is shown in the dorsal view.