Rescue of hematopoietic-marker expression. Sets of embryos were injected with either the control or xNAP1L MO (12 ng). Depleted embryos were subsequently injected at the 4-cell stage with 50 pg synthetic mRNA encoding SCL into the marginal zone or at the 8-cell stage into all 4 animal blastomeres with 25 pg synthetic mRNA encoding xNAP1L. The embryos injected with xNAP1L mRNA were allowed to develop until stage 18 and were then fixed and analyzed for SCL mRNA by in situ hybridization (A). The embryos injected with SCL mRNA were allowed to develop until stage 30 and then were fixed and analyzed for α-globin mRNA by in situ hybridization (B).