Cell-cycle–associated protein signatures. (A) Proteins (cyclin D₂/D₃, p27, and actin) extracted from freshly isolated naive T cells from a noninfected donor (lane 1), freshly isolated memory T cells from a healthy noninfected individual (CD8⁺ CD45RO⁺) (lane 2), and PBMCs isolated from a donor with AIM (lane 3) were subjected to Western blot analysis. (B) Western blot analysis and normalization ratio calculating the β-actin/antibody-positive protein band of activated EBV-specific T cells (lane 1) which were removed from TCR stimulus and maintained in either fresh medium alone (lane 2), IL-15 (lane 3), or IFN-α (lane 4) for 4 days. These results are representative of 3 separate experiments.