Genes up-regulated in vlt at 36 hpf represent myeloid genes. (A) Genes down-regulated in clo and scl^mo embryos, up-regulated in vlt, but unaffected in the other mutants, were linked to the myeloid lineage. Columns represent mutants of which name, tested allele, and the respective developmental stage are given; rows represent genes. Fold changes are shown as log2 (mutant/WT, or morphant/uninjected). Blue and yellow boxes indicate decreased and increased gene expression, respectively, in mutant compared with WT (morphant compared with uninjected) embryos. The expression of genes highlighted in red is further illustrated by in situ hybridization in panels B, C, D, and E. (B,C) Expression of slc22a4 in WT, clo^m39, and vlt^m651 embryos at 36 hpf; transcripts are highly restricted to myeloid cells in WT, abundant in circulating myeloid cells of vlt^m651 embryos, but absent in clo^m39 mutants. (D,E) In WT embryos, ncb5or is expressed in myeloid cells and in vasculature at 36 hpf. clo^m39 mutants show only low levels of transcription in angioblasts around the posterior region of the yolk tube extension (arrowheads). Panel D shows dorsal view; panel E shows lateral view.