Genes down-regulated in clo and scl^mo embryos show vascular expression pattern. (A) Expression profile of the vascular gene set. The set of genes specifically down-regulated in clo at 14 somites and in clo and scl^mo at 36 hpf was linked to the vasculature. Columns represent mutants of which name, tested allele, and the respective developmental stage are given; rows represent genes. Fold changes are shown as log2 (mutant/WT, or morphant/uninjected). Blue and yellow boxes indicate decreased and increased gene expression, respectively, in mutant compared with WT (morphant compared with uninjected) embryos. The expression of genes highlighted in red is further illustrated by in situ hybridization in panels B, C, D, and E. (B) Expression of dusp5 in WT and in clo^m39 embryos at 14 somites. Transcripts are found in ICM cells in WT, but are nearly absent in clo^m39 mutants; low levels of expression are only present in the tail bud. (C,D) Expression of dusp5 at 36 hpf. dusp5 transcripts are detected throughout the entire arterial and venous system in WT embryos. In clo^s5 embryos, expression is limited to a small area in the brain and to the posterior trunk (arrowheads). Panel C shows dorsal view; panel D shows lateral view. (E) mrc1 is expressed in the vasculature of WT embryos at 36 hpf, but is almost entirely absent in clo^m39.