Figure 4.
![Figure 4. NK cells induce a Ca2+ flux in iDCs followed by translocation of microtubule organizing center and IL-18 at the immunologic synapse. (A) NK cells were added to Fura2-AM-loaded iDCs and Ca2+ waves were monitored. Results are expressed as [Ca2+]i and represent the mean of calcium response of 15 different cells. (B) iDCs or iDC/NK conjugates after 3 hours of culture were stained for tubulin (green) or IL-18 (red). Nuclei are stained in blue by DAPI. Arrows point to the clustering of tubulin and IL-18 at the contact site between NK cells and iDCs.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/2/10.1182_blood-2004-10-3906/6/zh80140581310004.jpeg?Expires=1735497671&Signature=FvGaoVweXikSCqZ8x-RZ3y-YWg7XOnHG0qnEt8bRglKdTmnwxnKWWQOV9b-VcYnxwfd~zokwzThydJsJjs8NSS6CWsCIjI-rdsu2pmZcR7TUfJDwbOfoBgBY2fPkgqwKtwZT62f0yPNI69syToAUTyvXant9T95jkoQq38vfbUt~lc6rXWTZV~Sbv12R4woY91LgBkfFY4fqAKt7tYpyZKuKBp-vmz3Qwgce3UrOCiYigh2BHX~IMP~r0hFOLhWkSykJv8fgDxv8nuwZSUmWJBQeQxMizKvrqFcWAO01ezXHUw3cFOeLUWGRhfX~hb96Bu~dsy9Pb9sEVzmQqL3r3w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
NK cells induce a Ca2+ flux in iDCs followed by translocation of microtubule organizing center and IL-18 at the immunologic synapse. (A) NK cells were added to Fura2-AM-loaded iDCs and Ca2+ waves were monitored. Results are expressed as [Ca2+]i and represent the mean of calcium response of 15 different cells. (B) iDCs or iDC/NK conjugates after 3 hours of culture were stained for tubulin (green) or IL-18 (red). Nuclei are stained in blue by DAPI. Arrows point to the clustering of tubulin and IL-18 at the contact site between NK cells and iDCs.
