Transfusion of mHEL RBCs into HEL/CFA-immunized mice results in rapid removal of HEL⁺ RBCs. C57BL/6 mice were immunized with HEL/CFA. Two weeks after immunization, immunized mice and control unimmunized mice received transfusions of leukoreduced mHEL RBCs. Peripheral blood was harvested 6 hours later and stained with anti–mouse Ig to detect antibody-coated cells, which were visualized by flow cytometry (A-C). HEL⁺ cells were detected in peripheral blood from each group of animals by staining with anti-HEL followed by anti–mouse Ig (D-F). The experiments shown in this figure have been reproduced in at least 3 separate experiments. The data presented in this figure are representative results. Numbers indicate percent of events in gate.