Induction of chemotaxis of CD8⁺ T cells, but not CD4⁺ T cells or granulocytes, by poly(I:C)–stimulated BMMCs in vitro. BMMCs were preincubated in the absence (□) or in the presence of 10 μg/mL poly(I:C) (▦) for 48 hours. To analyze migration of CD4⁺ T cells (A) or CD8⁺ T cells (B), LN cells from OTII or OTI mice were used, respectively. Percentages of migrated CD4⁺ or CD8⁺ T cells were determined by FACS and expressed as “chemotactic index” (^**P < .001). One representative experiment of 3 is shown. Bone marrow cells were used to analyze migration of granulocytes. (C) Percentages of migrated granulocytes (Gr1⁺, CD11b⁺ double-positive cells) were determined by FACS. The “chemotactic index” was calculated as described in “Materials and methods.” One representative experiment of 3 is shown. BMMCs generated from 3 mice were analyzed in each experiment.