Effects of JNK-specific siRNA on basal proliferation and survival of B-lymphoma cells. (A) BKS-2 or (B) WEHI-231 B-lymphoma cells were cotransfected with the GFP plasmid and siRNA (control or JNK specific). Three days later, GFP⁺ cells were sorted by FACS and proliferation assay was performed as described in “Materials and methods.” Results are presented as means ± SE of triplicate cultures. (C) Hoechst analysis of GFP⁺ BKS-2 and WEHI-231 B-lymphoma cells or prostate cancer cells PC-3 cotransfected with control or JNK-specific siRNA. ▦ indicates G₂/M; □, S; ▨, G₁; and ▪, sub-G₁. (D) Western analysis of lysates from control or JNK-specific siRNA-treated WEHI-231 (top) and BKS-2 cells (bottom) by an antibody to JNK. The blots were then stripped and reprobed for β-actin as a loading control. Results are representative of 2 to 3 experiments. ^*P < .001 when response with JNK siRNA is compared with control siRNA.