Lentiviral shCCR2 results in reduced macrophage influx due to reduced CCR2 expression. (A) Macrophage recruitment to the peritoneum in mice that underwent transplantation with H1.shCCR2 lentivirus–transduced bone marrow is significantly reduced compared with those that received H1.Empty lentivirus–transduced bone marrow. Recruitment levels were found to be similar to control mice that underwent transplantation with Ccr2^–/– bone marrow (*P < .05, **P < .01). (B) CCR2 mRNA expression level in peritoneal macrophages of mice that underwent transplantation with H1.shCCR2-transduced bone marrow was reduced 7 weeks after transplantation (**P < .01, ***P < .001 compared with H1.Empty; #P < .01 compared with H1.shCCR2). (C) Ccr2 genotyping of transplanted C57Bl/6 mice, revealing a 360–base pair amplicon of wild-type Ccr2 in mice that underwent transplantation with H1.Empty- or H1.shCCR2 lentivirus–transduced bone marrow and the 290–base pair amplicon was observed in mice that underwent transplantation with Ccr2^–/– bone marrow. (D) Percent male bone marrow in bone marrow cell lysates from recipient mice, determined by PCR analysis on the Sry gene, located on the Y-chromosome. Error bars in panels A, B, and C represent SEM.