![Figure 2. Protein expression analysis of TRAIL, Bcl-2, Bcl-XL, FLIP, and FAS. U-266-1970 cells were treated with IL-6 (20 U/mL) alone or in combination with 1000 U/mL IFN-α or 1000 U/mL IFN-γ for the indicated times. (A) Western blot analysis. The cells were lysed and 20 μg total protein per lane was separated on NuPAGE 10% Bis-Tris (Bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane) gel and Western blot analysis was performed using specific antibodies targeting Apo2L/TRAIL, Bcl-2, Bcl-XL, FLIP, and β-actin. Three experiments were performed and one representative Western blot is shown. (B) FACS analysis. FAS surface expression was determined by flow cytometry using a primary antibody against Fas and a FITC-conjugated secondary antibody. The bars represent the fold induction of mean fluorescence intensity (MFI) as compared to cells treated with IL-6 alone. Data are expressed as mean ± SD, n = 3. The asterisks indicate significant differences to the corresponding control values (P < .05).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/4/10.1182_blood-2004-04-1322/7/zh80160582700002.jpeg?Expires=1735497497&Signature=GilBzJoMbVjwIf5H2x5MOQVDlg-RwM-5mv~WAF6rlkkBAab5VNtPm9b9yIsnxZZZhdUxojVKJn60VEeulcgfkwaweJ3qnfLlLhOdMp24uFZdn0ubn-Y4W9sd~Q5NiwMRfJzkAkww5S1ua66VpWhci9IauXrMFLcOzzKRJDDGJ7sGFJOtyYF-VYF81AAbViTUYGnxIv~8o~o4gQAo877MxAlVHKTb7-QznmOzk89FCtEUZqPd3A1Qw-TjKuHM-193HcDDYeC1mv0bcBDXdCUg0LXZmgwelBLD2GnC7mltRbT4QSAmPQsYqZX2inoHrb1cJmCT8bdvgwpDGghw7VtSAQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Protein expression analysis of TRAIL, Bcl-2, Bcl-XL, FLIP, and FAS. U-266-1970 cells were treated with IL-6 (20 U/mL) alone or in combination with 1000 U/mL IFN-α or 1000 U/mL IFN-γ for the indicated times. (A) Western blot analysis. The cells were lysed and 20 μg total protein per lane was separated on NuPAGE 10% Bis-Tris (Bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane) gel and Western blot analysis was performed using specific antibodies targeting Apo2L/TRAIL, Bcl-2, Bcl-XL, FLIP, and β-actin. Three experiments were performed and one representative Western blot is shown. (B) FACS analysis. FAS surface expression was determined by flow cytometry using a primary antibody against Fas and a FITC-conjugated secondary antibody. The bars represent the fold induction of mean fluorescence intensity (MFI) as compared to cells treated with IL-6 alone. Data are expressed as mean ± SD, n = 3. The asterisks indicate significant differences to the corresponding control values (P < .05).
