Figure 1.
![Figure 1. Time course of changes in phosphoinositide levels during fMLP stimulation of TNF-α-primed human neutrophils. Human neutrophils were labeled with [32P]Pi in the presence of 200 U/mL TNF-α, as described in “Materials and methods.” Neutrophils were washed, warmed for 5 minutes at 37°C in the presence or absence of 0.3 μM TGX-221, and then challenged with 100 nM fMLP or its vehicle for the times indicated. Incubations were terminated, lipids were extracted, and [32P] phosphoinositides were quantified as described in “Materials and methods.” Data are mean ± SD (n = 3-5) collected from separate preparations of neutrophils (where not shown, errors lie within the symbols).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/4/10.1182_blood-2005-03-0944/7/zh80160582600001.jpeg?Expires=1735537289&Signature=dkKtISTNXSVKaLKDWf3QfJnQIm9NCVOMuXjLajrCXgYuexvB78~P25TnwzMou-RvfHtz6DxmcOeiPP2C8mQXeps5Wpa-fkpSHzO6qlpkWoE-hmiADUWDsSXsMvtL5pMe-bXVvNZdxCQLu-PlGtqd2VR~Ouh2qQt11KAezJJQPY0~rQn4r~uXcXHbqltEya8Z5mU1ROXCdIxi3JqGJl9pHVBf4Zhsb08ligUElrPTwTfjeXVGZZWqcDmxapsBtw821~6Iict4tQPvLxvc3X5JpmbOniHiFJq6Orjz8IYlCGwvDhhdziZb1Ph1VheXTDMH3TWVWQ9vnlF8fdk0dXbiHg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Time course of changes in phosphoinositide levels during fMLP stimulation of TNF-α-primed human neutrophils. Human neutrophils were labeled with [32P]Pi in the presence of 200 U/mL TNF-α, as described in “Materials and methods.” Neutrophils were washed, warmed for 5 minutes at 37°C in the presence or absence of 0.3 μM TGX-221, and then challenged with 100 nM fMLP or its vehicle for the times indicated. Incubations were terminated, lipids were extracted, and [32P] phosphoinositides were quantified as described in “Materials and methods.” Data are mean ± SD (n = 3-5) collected from separate preparations of neutrophils (where not shown, errors lie within the symbols).
