HSV-2 infection induces death of macaque immature moDCs. Immature moDCs were exposed to HSV-2 or medium (Figure 1) and cells assayed after 4 hours or ON reculture. (A) Cell viability was determined by trypan blue exclusion. The mean percentages of viable cells (± SEM), relative to the input of cells replated after the 1-hour exposure to medium versus HSV-2, are shown from 9 to 17 experiments. (B) Overnight recultured immature macaque moDCs (medium-treated versus HSV-2–infected) were stained with annexin V and PI and analyzed by flow cytometry. The percentage of early apoptotic (annexin V–positive PI-negative, lower right quadrant) and late apoptotic/necrotic cells (annexin V–positive PI-positive, upper right quadrant) are indicated for 1 representative experiment of 6 performed. (C) The percentages of annexin V–positive PI-negative (left panel) and annexin V–positive (both PI-negative and PI-positive, right panel) cells are shown after 4 hours of reculture (medium-treated versus HSV-2–infected cells) and ON reculture (for cells exposed to medium [Med], infectious HSV-2 [HSV], or UV-treated HSV-2 [UV]). The minimal amounts of apoptosis seen in UV-treated HSV-2–exposed DCs above the medium controls could represent death induced by residual infectious virus or toxic effects of the virus stocks. Data were obtained using immature moDCs derived from 5 different Indian macaques (*DCs from T613 tested on 2 different occasions), 1 Chinese macaque (K453), and 3 human leukopacks (Hu 1, Hu 2, Hu 3; open symbols). Each symbol marks the individual donors. The black bars indicate the mean values.