Immature macaque DCs release cytokines and chemokines in response to HSV-2 infection. Cell-free supernatants were collected (after 4 hours or ON reculture) from infected (5 pfu per cell) versus uninfected (+ versus –) immature macaque moDC cultures. Cells from an additional 2 donors were also exposed to UV-treated HSV-2 (UV) and samples collected after overnight reculture. The presence of cytokines and chemokines was measured using the 22-plex Luminex assay (Upstate Biotechnology). The mean concentration of each cytokine/chemokine (mean pg/mL ± SEM) is shown from 3 to 17 experiments after 4 hours of reculture, from 15 to 21 experiments after ON reculture for uninfected versus infected DCs, and from 2 donors exposed to UV-treated HSV-2. Values for the CXCL8 responses from the 2 experiments comparing cells exposed to medium versus HSV-2 (live or UV treated) have been set at the 5000 pg/mL maximal value because the levels were out of range in the Luminex assay (with comparable MFIs for the differently treated cells). As a result, no error bars are shown for the UV-treated HSV-2 cell control.