Knockdown of ATP11a expression increases sensitivity to SCH66336. (A) Northern blot analysis of NIH3T3 cells transfected with nonspecific siRNA, or with 2 different siRNAs specific for murine ATP11a designated ASU and ASV. An arrow points to the 8-kb ATP11a mRNA. (Left) Ethidium bromide-stained gel; (right) autoradiogram. (B) Real-time RT-PCR to determine levels of ATP11a after transfection of cells with siRNAs. RNAs were isolated 36 hours after transfection. Values are relative to those of actin. Error bars indicate standard deviation. (C) Phase-contrast images of cells transfected with ASU or nonspecific siRNAs for 24 hours and subsequently exposed to 50 μM SCH66336 for 48 hours. Bars indicate 100 μm. The percentage of viable cells is indicated in the upper right-hand corners. The result shown is 1 of 2 independently performed experiments with similar results.