PCR analysis of buffy coat and CSF samples from affected triplets for HHV-8. Specific PCR product for ORF-26 of HHV-8 is shown (336-bp product; arrow).⁹  Lane 1, triplet B CSF sample; lane 2, triplet A buffy coat sample; lane 3, Mewo cell line (negative control); lane 4, BC-3 cell line (positive control); lane 5: DNA bp ladder.