Transfection of cells with GFP-PKC-δ reinstates PEP005 responsiveness. (A) KG1a cells were transiently transfected with EGFP-tagged PKC-δ, and expression of PKC-δ was confirmed by Western blotting after 24 hours. (B) PEP005 was added to cultures 24 hours after transfection and apoptosis was determined after 3 days in PEP005-treated EGFP-positive KG1a cells transfected with pEGFP empty vector (▪) or pEGFP-PKC-δ (□) using analysis of forward and side scatter profiles by FACS. Data are mean ± SD of 6 experiments. (C) Translocation of EGFP-tagged PKC-δ following treatment of KG1a cells with 200 nM PEP005 was monitored by confocal microscopy and shows translocation to the nuclear region of the cells within 5 minutes of treatment. Nuclei are counterstained with DAPI.