Tissue expression AMN and CUBN cDNA. Total RNA isolated from the designated tissues was amplified by RT-PCR. AMN primers were designed to amplify the full-length coding sequence (product size 1.4 kbp). CUBN primers amplified exons 8-12 (product size 0.5 kbp). PPID primers were used as a positive control for success of the RT reaction in each RNA sample (product size 0.6 kbp). Lanes: 1, kidney; 2, muscle; 3, spleen; 4, thymus; 5, liver; 6, heart; 7, lung; 8, pituitary; 9, testis; 10, cerebellum; 11, cerebrum; 12, placenta; 13, duodenum; 14, jejunum; 15, ileum; 16, colon; 17, pancreas.