Liver CD45⁺ SP tip cells can be generated from bone marrow (BM) SP or single stem cells after transplantation. FACS analysis of liver (Li) SP tip cells in lethally irradiated recipients that were transplanted with (A) 2 × 10⁶ unfractionated GFP⁺ marrow, (B) 200 purified bone marrow (BM) SP, or (C) single BM SP cells. CD45⁺ Li SP tip cells derived, in part, from donor (GFP⁺) marrow cells in each recipient. (D) CD45⁺ Li non-SP cells also derived from the transplanted single BM SP cell. Numbers in dot plots represent percentage of cells contained in each gate.