Expression of SPHK1 in murine, rat, and human tissues or cell lines. (A) Whole-cell lysates were subjected to Western blot analysis (WB) with antibodies directed against SPHK1 protein and α-adaptin as loading control. Among hematopoietic cell lines, IW1-32 and 745-A are erythroleukemic cell lines derived from Friend murine leukemia virus (F-MuLV)- or spleen focus-forming virus (SFFV)-induced erythroid tumor cells, 921 is an HS2 erythroleukemic cell line from spi-1-transgenic mice, N13 and N11 are macrophagic cell lines, FDC-P1 is a multipotent progenitor cell line, Ba/F3 is a pro-B-cell line, and HL-60 is a myeloblastic cell line. NIH3T3 and NRK (normal rat kidney) are fibroblastic cell lines. (B) Western blot with anti-SPHK1 antibodies of whole-cell lysates harvested 24 hours after transfection with Luc siRNA and SPHK1 siRNA. HeLa cells are epithelial cells.