Analysis of mice reconstituted with FLAG-CrmA-GFP retrovirus-infected (E14) fetal liver cells. (A) Cells were harvested from mice 8 weeks after reconstitution and stained with mAbs to cell lineage-specific markers and analyzed by FACS. GFP expression was compared between mice reconstituted with fetal liver cells infected with FLAG-CrmA-GFP–expressing virus or control (GFP alone) virus. Numbers in quadrants represent percentages of cells within each staining profile. (B) Lysates from cells of hemopoietic organs were used for Western blot analysis using anti-FLAG mAb to assess levels of FLAG-CrmA protein expression. Results are representative of more than 6 analyses.